Fig. 7: Mammary transgenic expression of p27CK-DD increases mammary duct branching, mammary hyperplasia and yields mammary cancers that generate liver metastases. | Nature Communications

Fig. 7: Mammary transgenic expression of p27CK-DD increases mammary duct branching, mammary hyperplasia and yields mammary cancers that generate liver metastases.

From: C-terminally phosphorylated p27 activates self-renewal driver genes to program cancer stem cell expansion, mammary hyperplasia and cancer

Fig. 7: Mammary transgenic expression of p27CK-DD increases mammary duct branching, mammary hyperplasia and yields mammary cancers that generate liver metastases.

a Representative mammary gland whole mounts stained with Carmine red from MMTVCre controls and MMTV-Cre X TGp27CK- (p27CK-) and MMTV-Cre X TGp27CK-DD (p27CK-DD) mice show greater duct branching (top 2 panels) and hyperplasia (bottom panels) in the p27CK-DD bigenics. b Duct hyperplasia was quantitated from whole mounts as described and graphed as mean %+/−SEM. p values were calculated by one-way ANOVA with post hoc 2 by 2 comparisons using Tukey correction. N = 8 mice for MMTVCre controls, N = 7 mice for TGp27CK-, N = 6 mice for TGp27CK-DD (See also SFig. 6b for quantitation of duct branching). c The number of mice with mammary cancers over the total number evaluated for each genotype is graphed as %. p values calculated by Fisher’s Exact Test are shown. N = 14 mice for MMTVCre controls, N = 14 mice for TGp27CK-, N = 14 mice for TGp27CK-DD. d FFPE mammary glands from the indicated genotypes were stained with hematoxylin and eosin (H&E) or with antibodies to p27pT198, to cytokeratin or to myoepithelial marker, p63, as indicated and representative photomicrographs shown. e Livers show representative micrometastases from mammary cancer-bearing MMTV Cre X p27CK-DD mouse 1668 (see also Fig. S6). Source data are provided as a Source data file.

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