Fig. 6: BFPVNs of the HDB innervate the limbic system.
From: Parvalbumin-expressing basal forebrain neurons mediate learning from negative experience
a Top left, schematic illustration of cell-type specific anterograde tracer virus injection into the HDB. Top right and middle, fluorescent image of the injection site (green, eGFP). Bottom, normalized BFPVN axon density in target brain areas. Bars and errorbars indicate the mean ± SEM from n = 3 mice. b Top left, schematic illustration of the medial septum on a coronal atlas image. Top right, fluoromicrograph of labeled axons in the MS (repeated in n = 3 mice). Middle and bottom left, fluorescent images of ChAT+ and PV+ target neurons in the medial septum (white arrowheads indicate plausible contact sites). Right, electronmicrographs of synaptic contacts established by BFPVNs with the target cell types shown in the left panels (different examples). HDB BFPVN axons are labeled by DAB precipitate (black asterisk); black arrows indicate synaptic contacts. c, Top left, schematic illustration of the hippocampus on a coronal atlas image. Top right, fluoromicrograph of labeled axons in the CA1 (repeated in n = 3 mice). Middle and bottom left, fluorescent images of CR+ and PV+ target neurons in the hippocampus. Middle and bottom right, electronmicrographs of synaptic contacts established by BFPVNs with the target cell types shown in the left panels. d Top left, schematic illustration of the retrosplenial cortex (RSC) on a coronal atlas image. Top right, fluoromicrograph of labeled axons in the RSC (repeated in n = 3 mice). Bottom left, fluorescent images of a PV+ target neuron in the retrosplenial cortex. Bottom right, electronmicrograph of a synaptic contact established by BFPVNs with the target cell type shown on the left. VDB ventral limb of the diagonal band of Broca, MS medial septum, RsG retrosplenial cortex, granular part, Hipp hippocampus, MMn meidal mamillary nucleus, SUM supramamillary nucleus, PT/sm paratenial thalamic nucleus/stria medullaris, MO medial orbital cortex, LSi lateral septum, intermediate part, IL/DP inflalimbic cortex/dorsal peduncular cortex, MRR median raphe region, VTA ventral tegmental area. Source data are provided as a Source Data file.
