Fig. 6: The level of phospholipids is globally elevated in a ΔplpD strain.
PA14 (WT) and SEH88 (ΔplpD) cultures were grown in parallel in LB and washed in PBS before the cells were lysed and prepared for a lipidomic analysis using LC/MS. a A principal component analysis (PCA) of the lipid profiles is shown. PA14 profiles are shown in red and SEH88 profiles are shown in green. b An unpaired, parametric Welch t-test (two-sided) with unequal variance was used to generate a volcano plot. Lipids that were present at a higher level in SEH88 are shown in red and lipids that were present at a lower level in SEH88 are shown in blue. Log 2(FC) refers to log2 for fold change (FC) with a cut-off line for FC ≥ 1.2; -log(p) refers to -log10 for p-values with a cut-off line for p-value ≤ 0.05. c A heatmap showing the phospholipids that were differentially produced in PA14 and SEH88 cultures was generated using Pearson-Ward correlation analysis. The level of the differential for each lipid is shown in Table S9, a detailed list of the LC/MS mass assignments is shown in Table S10, and a heatmap showing the top 70 lipids that were differentially produced is shown in Fig. S14. The PCA, volcano plot and heat map were generated using MetaboAnalyst 5.070. Abbreviations: cardiolipin (CL); fatty acid (FA); oxidixed fatty acid (FA-O); monoacylated glycerophospholipid (Lyso); monoglycerol (MG); phosphatidic acid (PA); phosphatidylethanolamine (PE); phosphatidylglycerol (PG); phosphatidylserine (PS).
