Fig. 2: Spa2(1-535) regulates actin cable remodeling upon ES.
From: Spa2 remodels ADP-actin via molecular condensation under glucose starvation
a Intrinsically disordered region analysis. b Representative fluorescent images of GFP-tagged Spa2 truncation variants upon ES under the indicated conditions. Scale bars, 2 μm. c, d Quantification of the mean signal intensity of Spa2(1-535)-GFP filaments and puncta of Spa2(281-535)-GFP upon ES (n = 14, 19, 20 and 20 cells for each condition). The dashed line indicates 1. e Localization of Abp140-3×GFP in Spa2 truncating mutants, with or without ES at 5 min. Scale bars, 2 μm. f, g Quantification of the mean intensity of Abp140-3×GFP per filament and total signal intensity per indicated truncated Spa2 mutant cells in the presence or absence of ES (from left to right: n = 266, 207, 299, 327, 166, 150 actin filaments for f; n = 32, 30, 34, 35, 20, 21 cells for g). The dashed line indicates 1. Means, SDs, and P-values were calculated using GraphPad Prism 6. Significance between two sets of data were determined by the one-way analysis of variance (***p < 0.001, ****p < 0.0001, **p < 0.01 and specified and ns = not significant). Source data is provided as Source Data file.
