Fig. 1: The workflow of ID-organic cytoMS. | Nature Communications

Fig. 1: The workflow of ID-organic cytoMS.

From: In-depth organic mass cytometry reveals differential contents of 3-hydroxybutanoic acid at the single-cell level

Fig. 1

The cell suspension was pumped into an ordering capillary to achieve single-cell monodispersion, and intact single cells were lysed online after they left the ordering capillary and mixed with sheath liquid via ice-bath ultrasonication. Segmented lysate from single cells was subsequently analyzed by non-contact ESI-MS. Extended analysis time ensures that abundant MS/MS data are obtained and isomer discrimination occurs at the single-cell level.

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