Fig. 4: Qualitative and quantitative analysis of isomers at single-cell level. | Nature Communications

Fig. 4: Qualitative and quantitative analysis of isomers at single-cell level.

From: In-depth organic mass cytometry reveals differential contents of 3-hydroxybutanoic acid at the single-cell level

Fig. 4

Single-cell MS2 spectra of ions at a m/z 744.5543 (PC (15:1_18:1) and PE (18:1_18:1)); b m/z 118.0863 (5-aminovaleric acid and valine); and c m/z 103.0401 (4-hydroxybutanoic acid and 3-hydroxybutanoic acid). (Characteristic fragment ions and corresponding MS peaks are highlighted in orange or green). The relative abundance of the above isomers among 60 single cells is shown in d–f. The relative isomer ratio was defined as the intensity ratio between the characteristic fragment ions. (m/z 184.0733 vs. m/z 603.5352; m/z 101.0597 vs. m/z 72.0808; m/z 59.0138 vs. m/z 57.0345). Source data are provided as a Source Data file for a–f.

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