Fig. 5: Clustering of MCF-7 cells with BHB/GHB isomer information. | Nature Communications

Fig. 5: Clustering of MCF-7 cells with BHB/GHB isomer information.

From: In-depth organic mass cytometry reveals differential contents of 3-hydroxybutanoic acid at the single-cell level

Fig. 5

a t-SNE visualization of 4 types of tumor cells. b Uniform manifold approximation and projection (UMAP) visualization of MCF-7 cells with and without BHB/GHB isomers (n = 138 cells). c Relative abundance of BHB/GHB isomers (MS peak intensity at m/z 59.0138 vs. m/z 57.0345) among 3 clusters (n = 138 cells in total). The boxplot shows the median and 0.25 and 0.75 quantiles, and the whiskers extend to points within the 1.5 interquartile range of the lower and upper quartiles. (Kruskal–Wallis test, P = 4.3E-23, *** denotes P < 0.001). Source data are provided as a Source Data file for c.

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