Fig. 6: Altered structure and function of cortex in male BPA-exposed and ArKO mice. | Nature Communications

Fig. 6: Altered structure and function of cortex in male BPA-exposed and ArKO mice.

From: Male autism spectrum disorder is linked to brain aromatase disruption by prenatal BPA in multimodal investigations and 10HDA ameliorates the related mouse phenotype

Fig. 6: Altered structure and function of cortex in male BPA-exposed and ArKO mice.The alternative text for this image may have been generated using AI.

A Golgi staining showed shorter apical and basal dendrites in male BPA-exposed (apical: n = 36, β =-350μm, 95% CI [−679, −20], P = 0.04; basal: n = 36, β = −217, 95% CI [−315, −119], P = 1.4 × 10−5) and ArKO mice (apical: n = 35, β = −541.9, 95% CI [−666, −417], P = 1.3 × 10−17; basal: n = 35, β = −163, 95% CI [−308, −17], P = 0.02) compared to male vehicle (apical n = 35, basal n = 36) or WT(apical n = 36, basal n = 36). B Golgi staining showed male BPA-exposed (apical: n = 186, β = −4.7, 95% CI [−9.2, −0.2], P = 0.04; basal: n = 40 β = −6.7, 95% CI [−16, 2.8], P = 0.17) and ArKO (apical: n = 148 β = −4.4, 95% CI [−7.7, −1.0], P = 0.01; basal: n = 51 β = −5.2, 95% CI [−14.4, 4.1], P = 0.27) mice had lower spine densities on apical but not on basal dendrites vs. vehicle (apical n = 189, basal n = 56) or WT mice (apical n = 185, basal n = 55). For golgi staining experiments, 3 mice/group with 9–12 neuron measures/mouse. Spine count datapoints represents the number of spines on a single 10 μm concentric circle. C Representative photomicrographs of golgi stained cortical neurons, scale bar is 100μm. D Electrocorticograms (ECoG) revealed an increased in the average spectral power at 8 Hz in BPA-exposed (n = 4; *a 8 Hz MD = −0.5; t(325) = 3.4 P = 0.01) mice and (E) 4–6 Hz in ArKO mice (n = 4; *b 4 Hz MD = −0.2; t(120) = 4.3, P = 0.0006; *c 5 Hz MD = −0.2, t(120) = 6.1, P < 0.0001; *d 6 Hz MD = −0.2, t(120) = 5.2 P < 0.0001) vs. vehicle (n = 7) or WT (n = 4)mice. Generalized estimating equations were used clustering by mouse (Panels A, B) and assuming an exchangeable correlation structure. For (D) and(E) Independent t test were used used, P-values were corrected for multiple comparisons using Holm-Sidak. All statistical tests were two-sided. Plots show mean ± SEM. Source data are in a Source Data file.

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