Fig. 1: Antagomir treatment of 3D human umbilical vein endothelial cell (HUVEC) microvessel cell cultures.
From: Space radiation damage rescued by inhibition of key spaceflight associated miRNAs
a, b Quantification of formation of microvessels using 3D cultures of human microvessels irradiated with 0.5 Gy of simplified simulated galactic cosmic rays (GCR) compared to sham irradiated samples. Irradiated cultures were treated with or without antagomir-induced inhibition of miR-125b-5p, miR-16-5p, and/or let-7a-5p. For the boxplots the center line represents the median and the lines extending from both ends of the box indicates the quartile (Q) variability outside Q1 and Q3 to the minimum and maximum values. a mature and b angiogenesis microvessels. The p-values were determined by two-side multiple pairwise comparison. c Mature and angiogenesis microvessels fixed and fluorescently stained with 5-(4,6-dichlorotriazinyl) aminofluorescein (DTAF) after GCR irradiation, with or without antagomirs 24 h prior to irradiation. Scale bar = 100 µm. For mature the following biological independent samples were used: n = 17 for 0 Gy, n = 18 for 0.5 Gy, n = 17 for 0.5 Gy + 3 antagomirs, n = 17 for 0.5 Gy + let-7a-5p antagomir, n = 17 for 0.5 Gy + miR-16-5p antagomir, and n = 16 for 0.5 Gy + miR-125b-5p antagomir. For angiogenesis the following biological independent samples were performed: n = 18 for 0 Gy, n = 20 for 0.5 Gy, n = 14 for 0.5 Gy + 3 antagomirs, n = 11 for 0.5 Gy + let-7a-5p antagomir, n = 14 for 0.5 Gy + miR-16-5p antagomir, and n = 10 for 0.5 Gy + miR-125b-5p antagomir.
