Fig. 4: Global transcriptomic analysis reveals that antagomirs revert gene profiles closer to control samples.
From: Space radiation damage rescued by inhibition of key spaceflight associated miRNAs
a Schematic of the experimental profile for the mature and angiogenesis 3D cell culture models irradiated with 0.5 Gy of GCR and prepared for RNA-sequencing. There was n = 5 biological replicates for each condition. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. b Principal Component Analysis (PCA) of the significantly regulated genes (p-value < 0.05) for all conditions compared with likelihood ratio test (LTR) analysis for both mature and angiogenesis 3D microvessel cell culture models. c Heatmap of significantly regulated genes (p-value < 0.05) for mature and angiogenesis cell culture models. For each gene (i.e. row), the criteria for display was to have at least one comparison per gene to be significantly regulated. Then the trends for non-significant genes for that row were also displayed. The log2(fold-change) values are displayed. The side color bars indicate the number of significant genes that are either up- or down-regulated per row and also the type of gene. d Volcano and e MA plots for each comparison. f Upset plot displaying the overlapping significantly regulated genes (p-value < 0.05) for mature and angiogenesis models with and without antagomir treatment. For all RNA-seq data Wald test and the likelihood ratio test was used to generate the F statistic p-value. The adj. p-value plots are also provided in Supplementary Fig. 3.
