Fig. 6: Expression of 21 genes rescued by the antagomir treatment in astronauts from the NASA Twin Study, JAXA CFE, and Inspiration4 (i4) missions.
From: Space radiation damage rescued by inhibition of key spaceflight associated miRNAs
a–d Heatmaps displaying the 21 key gene profile for the different cell types (i.e. CD4 T cells, CD8 T cells, CD19 B cells, and lymphocyte deleted cell population) from RNA-seq on the NASA Twin Study. e Heatmap of the normalized plasma cell-free RNA expression values for the 21 key genes over time for the six astronauts over 120 days in space from JAXA study. The values shown are the averaged normalized expression values for all six astronauts for each time point during flight and post-flight. The three pre-flight time points were averaged together, since the changes for genes in the time leading up to flight are considered to be the same and part of the baseline values. For the time, L = Launch (i.e. meaning time after launch from Earth and length in space) and R = Return to Earth. f A schematic of the i4 experimental design created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license. Global gene expression profile of the g 21 key genes and h all genes from scRNA-sequence data from the i4 astronauts. i–r Heatmaps displaying the 21 key gene profile for the different cell types (i.e. PBMCs, CD4 T cells, CD8 T cells, Other T cells, B cells, CD14 cells, CD16 cells, NK cells, Dendritic cells (DC), and other cells) from scRNA-seq on the i4 astronauts. s Heatmap visualization of the 21 key genes on the i4 astronauts of relative expression changes in the postflight relative to preflight skin biopsy sample across four skin compartments including Outer Epidermis (OE), Inner Epidermis (IE), Outer Dermis (OD), and Vasculature (VA).
