Fig. 8: Pathway analysis on the mature and angiogenesis 3D microvessel cell culture models revealing key functions being mitigated by the antagomirs after exposure to GCR irradiation.
From: Space radiation damage rescued by inhibition of key spaceflight associated miRNAs
Lollipop plots showing Gene Set Enrichment Analysis (GSEA) pathway analysis of the Hallmark pathways for a mature and b angiogenesis 3D cell culture models. The right of each plot displays circular nodes comparing 0.5 Gy versus 0 Gy (left side of node) with 0.5 Gy plus antagomir treatment versus 0 Gy (right side of node) analyzed with the Cytoscape plugin Enrichment Map. Only significantly regulated pathways with FDR < 0.25 are shown. GSEA Reactome pathway analysis of RNA-seq data on c mature and d angiogenesis 3D cell culture models displaying the pathways that are significantly altered by 0.5 Gy irradiation with or without antagomir treatment compared to 0 Gy. Gene sets that were significantly enriched in the same direction for the two treatment groups (both up or both down) are not shown leaving only opposing or single pathway enrichments. Only significantly regulated pathways with FDR < 0.05 are shown. The nominal enrichment score (NES) represents the relative degree a gene set is changed and is corrected for gene set size.
