Fig. 2: Characterization and catalytic action of ARG-16.
From: Emergence of a short peptide based reductase via activation of the model hydride rich cofactor
a Schematic representation of ARG-16 nanostructures, b fluorescence spectra of ThT in the presence and absence of ARG-16, c TEM micrograph of ARG-16, inset: zoomed portion shows the hollow interior of ARG-16 nanotubes indicated by red arrows. The experiment was repeated for at least three times. d Representative zeta potential plot of ARG-16 and GLU-16 assemblies (the experiment was repeated for three times), e bar diagram of the yield of alcohol (2) by system and controls in 12 h, f CLSM micrograph of FITC bound ARG-16, g Time-dependent HPLC traces of reaction mixture of reduction, inset showing time course reaction yield by ARG-16-NaBH4 system. The error bars are calculated from three independent experiments. All data are presented as the mean ± s.d. (n = 3 separate experiments). For the bar diagram, statistics were estimated using a two-sided homoscedastic ‘t’ test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001; exact p value for NaBH4-1 system is 0.00037, ARG-16-1 system is 0.00031 and for the system with only 1 is 0.00031) in comparison with the ARG-16-NaBH4-1 system as reference. Source data are provided as a Source Data file.
