Fig. 3: Sema restores mitochondrial dysfunction and morphology in pathological cardiac remodeling. | Nature Communications

Fig. 3: Sema restores mitochondrial dysfunction and morphology in pathological cardiac remodeling.

From: Semaglutide ameliorates cardiac remodeling in male mice by optimizing energy substrate utilization through the Creb5/NR4a1 axis

Fig. 3: Sema restores mitochondrial dysfunction and morphology in pathological cardiac remodeling.The alternative text for this image may have been generated using AI.

A Heatmap showing the expression profile of mitochondrial respiratory chain signaling pathway gene sets in mouse cardiac tissue (n = 3). B TEM images of mouse cardiac tissues and quantification of the mitochondrial sectional area (scale bar: 2μm/1μm; n = 6). C The quantification of fragmented fused mitochondria (n = 6); Mitochondrial area: F (3, 20) = 109.4, P = 1.4e-012; Cristae/Mitochondrial area: F (3, 20) = 84.57, P = 1.6e-011. D The quantification of mitochondrial abundance by mitochondrial number and mtDNA/nDNA (n = 6); Mitochondrial number; F (3, 20) = 9.328, P = 4.6e-004; MtDNA/nDNA: F (3, 20) = 21.25, P = 2.0e-006. E Western blotting was used for protein quantitative analysis of mitochondrial function- and structure-related marker proteins Drp1, Opa1, Mfn2, Tom20, COX IV, SDHB, NDUFV2, and ATP5A1 and normalized to VDAC (n = 6 independent experiments with similar results); F (24, 160) = 67.22, P = 4.5e-071, F (3.243, 64.85) = 126.4, P = 8.4e-028, F (3, 20) = 72.44, P = 6.4e-011, F (20, 160) = 0.9693, P = 5.0e-001. F PCR was used for mRNA quantitative analysis of mitochondrial function- and structure-related marker proteins Drp1, Opa1, Mfn2, Tom20, COX IV, SDHB, NDUFV2 and ATP5A1 and normalized to VDAC (n = 6); F (24, 160) = 53.30, P = 6.3e-064, F (4.911, 98.21) = 49.04, P = 5.4e-025, F (3, 20) = 22.95, P = 1.1e-006, F (20, 160) = 1.417, P = 1.2e-001. All results are shown as the mean ± SEM, and analysis using one-way ANOVA followed by Bonferroni post hoc test (C-F) was conducted. p values are indicated. Source data are provided as a Source Data file. TEM, transmission electron microscopy; mtDNA, mitochondrial DNA; nDNA, nuclear DNA.

Back to article page