Fig. 4: AAV.FUS candidates show improved neuronal tropism.
From: Engineering viral vectors for acoustically targeted gene delivery
a All AAV.FUS candidates show improved neuronal tropism upon FUS-BBBO gene delivery. AAV.FUS.3 had 56% more likelihood of transducing a neuron than AAV9 (69.8%, vs 44.7% neuronal transduction, respectively; (for all samples p < 0.0001, one way ANOVA, F(5, 31) = 52.60, P < 0.0001; n = 8 for AAV9, n = 6 for all AAV.FUS.1,3,4,5, n = 5 for AAV.FUS.2, center for the error bars represents arithmetic mean.). b Representative images showing AAV9 transducing both neurons (blue, NeuN staining, example neurons designated by an arrow) and non-neuronal cells (example non-neuronal cells designated by an arrowhead). c In comparison, more of the cells transduced with AAV.FUS (green) are neurons (example neurons designated by an arrow), rather than non-neuronal cells (example cell designated by an arrowhead). IV injection dose, 1010 vg/g. Scale bars are 50 μm. (****p < 0.0001). Error bars are 95% CI.
