Fig. 1: Global strategy for discovering AmDHs among the biodiversity.

This includes five main steps: 1) environmental sampling to define the reference AmDH family (ref-AmDHs) and the NAD(P)-dependent enzyme pool, 2) sequence clustering, 3) cluster analysis including the search for distant homologs by HMM-HMM profile comparison, 4) selection of candidate enzymes and 5) production and in vitro tests of selected enzymes.