Table 2 Analytical yields and enantiomeric excess in 3C-amines 5b, 12b-13b

From: A refined picture of the native amine dehydrogenase family revealed by extensive biodiversity screening

 

hexan-3-amine (5b)

heptan-3-amine (13b)

hexan-2-amine (12b)

 

conv. (%)

ee (S) (%)

conv. (%)

ee (S) (%)

conv. (%)

ee (S) (%)

MGYP000211951848

45.5 ± 4.8

53.3 ± 0.4

23.7 ± 2.7

− 29.3 ± 3.7

88.3 ± 0.4

96.3 ± 0.2

MGYP001209562846

50.1 ± 2.3

68.6 ± 2.3

41.5 ± 2.5

−25.4 ± 3.4

85.9 ± 0.0

95.2 ± 0.2

A0A229HGK2

63.3 ± 2.7

>99.9

96.5 ± 8.0

99.9 ± 0.1

99.1 ± 0.0

99.4 ± 0.1

A0A1Q4UXH9

61.2 ± 0.1

>99.9

30.7 ± 0.7

93.4 ± 0.1

98.6 ± 0.3

99.0 ± 0.1

A0A365ZD63

35.2 ± 1.8

79.8 ± 0.1

6.5 ± 0.1

37.9 ± 1.3

96.4 ± 0.6

98.9 ± 0.1

A0A646KJR1

43.8 ± 2.2

99.0 ± 0.2

44.2 ± 0.3

88.3 ± 0.5

96.3 ± 0.2

96.4 ± 0.1

MicroAmDH

65.3 ± 0.6

96.2 ± 0.1

13.4 ± 1.2

96.9 ± 0.2

94.7 ± 0.0

87.6 ± 0.1

PortiAmDH

25.2 ± 0.6

64.8 ± 1.4

9.3 ± 0.6

− 25.5 ± 1.5

88.9 ± 1.4

96.6 ± 0.1

CfusAmDH

7.8 ± 0.4

38.0 ± 1.3

0.1 ± 0.1

− 38.2 ± 0.3

86.2 ± 0.0

97.7 ± 0.1

  1. nd not detected. Reactions conditions: 10 mM substrate, 2 M NH4HCO2 buffer, pH 9.0, 0.2 mM NADP+, 0.2 mM NAD+, 11 mM glucose, 3 U ml−1 GDH-105, 1.0 mg ml−1 purified enzyme, 24 h, 30 °C. Analytical yields in amines and ee were obtained after derivatization with BzCl and FDAA respectively, and UHPLC-UV analysis (conditions 1) (see Methods). Uncertainties represent the range of values obtained with two independent experiments. Chromatogram and calibration curves are given in Supplementary Figs. 14 and 15. Source data are provided as a Source Data file.
Back to article page