Fig. 3: Gene expression processes of two types of CssDNA vectors in the CFE system. | Nature Communications

Fig. 3: Gene expression processes of two types of CssDNA vectors in the CFE system.

From: Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems

Fig. 3: Gene expression processes of two types of CssDNA vectors in the CFE system.

a, d EGFP fluorescence intensity of different concentrations of CssDNA(+) or CssDNA(−) vector produced at the protein expression plateau, compared to the corresponding CssDNA in the presence of aphidicolin. b, e Fluorescence intensity of EGFP produced by different concentrations of CssDNA(+) or CssDNA(−) when only T7 complementary strands are present or when T7 complementary strands coexist with aphidicolin. All fluorescence signals in a and b, d and e were normalized based on the average fluorescence of the corresponding CssDNA expression plateau at a concentration of 2 ng/μL. Data collected in a, b, d and e were monitored by a microplate reader and are presented as mean ± standard deviation (s.d.) for n = 3 biologically independent experiments, individual data points are overlaid, source data provided. c, f The trend of protein expression level changes with the concentration of CssDNA(+) or CssDNA(−) vectors. g Schematic representation of the different protein expression pathways of CssDNA(+) and CssDNA(−) vectors.

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