Fig. 4: Construction of logic gates using CssDNA as a logic element. | Nature Communications

Fig. 4: Construction of logic gates using CssDNA as a logic element.

From: Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems

Fig. 4: Construction of logic gates using CssDNA as a logic element.

a A two-input logic gate was constructed using CssDNA(−) as the logic element and protein as the output. b Schematic illustration of the addition of aphidicolin to reduce the background signal from CssDNA(-) self-expression and to improve the signal-to-noise ratio of the logic gates. c-e Schematic and fluorescence signals of two-input logic gates under different input combinations, including OR, INHIBIT (INH) and NOR. All fluorescence signals in c, d and e were normalized according to the fluorescence intensity of the corresponding initial gate structure expression plateau under no input conditions. Data collected in c, d and e were monitored by a microplate reader and are presented as mean ± standard deviation (s.d.) for n = 3 biologically independent experiments, individual data points are overlaid, source data provided.

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