Fig. 2: Difference density maps for bound nucleotides in mBcs1L in the ADP and ATP states.

A Experimental procedure used to prepare EM grids. B Depiction of bound ATP-Mg²⁺ at nucleotide-binding sites for individual subunits of the structure of ATP-bound mBcs1L determined with C1 symmetry. For each panel, the protein is shown as a ribbon diagram, the bound ATP is rendered as a stick model, and the Mg²⁺ is given as a green ball. Difference EM density generated in Phenix omitting ATP is shown as a gray wire cage and contoured at 15σ level. The middle large panel shows the density of the ATP molecule after the seven-fold symmetry was imposed. C Difference EM density shown as wire cages and contoured at 10σ level for the ADP molecules bound at each individual nucleotide-binding site in the structure of mBcs1L determined with C1 symmetry in the presence of ATP and ISP-ED. The middle large panel shows the density of the ADP molecule after the seven-fold symmetry was imposed.