Table 2 RNA in situ hybridization T probes for use in the RNAscope assay
From: Single-cell and spatially resolved interactomics of tooth-associated keratinocytes in periodontitis
Target | Channel | Wavelength | NBCI ref sequence | Chemistry | Cat. # |
|---|---|---|---|---|---|
KRT19 | T1 | 488 | NM_002276 | V1 | 310221-T1 |
CCL28 | T1 | 488 | NM_148672.2 | V2 | 418001-T1 |
Polr2a (+control) | T1 | 488 | NM_000937.4 | V1 | 324311 |
Krt19 | T1 | 488 | NM_008471.2 | V2 | 402941-T1 |
LAMB4 | T2 | 550 | NM_001318048.1 | V1 | 530651-T2 |
CXCL17 | T2 | 550 | NM_198477.2 | V2 | 513241-T2 |
P. gingivalis | T2 | 550 | NR_040838.1 | V2 | 532132-T2 |
PPIB (+control) | T2 | 550 | NM_000942.4 | V1 | 324311 |
CXCL8 | T3 | 647 | NM_000584.3 | V2 | 573621-T3 |
Fusobacterium | T3 | 647 | NR_026083.1 | V2 | 433881-T3 |
UBC (+control) | T3 | 647 | NM_021009 | V1 | 324311 |
CXCL14 | T4 | 750 | NM_004887.4 | V1 | 425291-T4 |
EB-16S | T4 | 750 | * | V2 | 464461-T4 |
HPRT1 (+control) | T4 | 750 | NM_000194.2 | V1 | 324311 |
SAA1/2 | T5 | 488 | NM_000331.6 | V1 | 813351-T5 |
IL36G | T5 | 488 | NM_001278568.1 | V2 | 424791-T5 |
COL7A1 | T6 | 550 | NM_000094.3 | V1 | 803381-T6 |
RHOV | T6 | 550 | NM_133639.3 | V1 | 532931-T6 |
LGR6 | T6 | 550 | NM_001017404.1 | V1 | 410461-T10 |
IL1B | T6 | 550 | NM_000576.3 | V2 | 310361-T6 |
NPPC | T7 | 647 | NM_024409.2 | V1 | 431221-T7 |
CCL20 | T7 | 647 | NM_001130046.1 | V2 | 409611-T7 |
ODAM | T8 | 750 | NM_001385579.1 | V1 | 300040-T8 |
PAPPA | T8 | 750 | NM_002581.5 | V1 | 582481-T8 |
IL1A | T8 | 750 | NM_000575.3 | V2 | 556791-T8 |
SFRP1 | T9 | 488 | NM_003012.4 | V1 | 429381-T9 |
TNFSF15 | T9 | 488 | NM_005118.3 | V2 | 403121-T9 |
IL18 | T10 | 550 | NM_001562.3 | V1 | 400301-T10 |
IL6 | T10 | 550 | NM_000600.3 | V2 | 310371-T10 |
RHCG | T11 | 647 | NM_001321041.2 | V1 | 834511-T11 |
FDCSP | T11 | 647 | NM_152997.3 | V1 | 444231-T11 |
CXCL3 | T11 | 647 | NM_002090.3 | V2 | 1002151-T11 |
NEAT1 | T12 | 750 | NR_131012.1 | V1 | 411541-T12 |
SOX6 | T12 | 750 | NM_017508.2 | V1 | 524791-T12 |
CXCL1/2 | T12 | 750 | NM_001511.4x | V2 | 427151-T12 |
DapB (- control) | T1-T4 | various | EF191515 | V1 | 324341 |
