Fig. 6: Simulations and experimental validations of metabolic interactions in two-member consortia.
From: Engineering natural microbiomes toward enhanced bioremediation by microbiome modeling
Predicted metabolic interactions between strains 7D-2 and X-1 in single-culture (a) and co-culture (b) by community modeling. Ten testable predictions were provided for experimental verification of the simulations. The letters in parentheses following the predictions refers to the panels c–g supporting the corresponding predictions. BO bromoxynil octanoate, DBHB 3,5-dibromo-4-hydroxybenzoate; BRO bromoxynil. c, d Comparison of cell growth of 7D-2 and X-1 growing separately versus in co-culture. The log transformed colony-forming unit (CFU) of each strain cultured in medium with BO (b) or bromoxynil (c) as the sole carbon source is shown. The data are presented as mean values ± SD (n = 3 biological independent replicates). e-g The degradation ability of BO by X-1 (e), 7D-2 (f) and co-cultures (g). The concentrations of BO and bromoxynil produced during BO degradation in the medium with BO as the sole carbon source are showed. The data are presented as mean values ± SD (n = 3 biological independent replicates). Source data are provided as a Source Data file.
