Fig. 5: Predictions of fibrillation-prone regions of the CT sequence.
From: Structural conversion of the spidroin C-terminal domain during assembly of spider silk fibers

The CT sequence with the positions of the helices in the model of the soluble CT shown as comparison. Amino acid residues with β-sheet chemical shifts from ssNMR are indicated in red. Computational analysis using ZipperDB fibrillation propensity and theoretical inter-sheet distances in combination with TANGO cross-β aggregation at pH 7 and pH 4 suggest hotspots for possible formation of β-sheets.