Table 1 The calculation times for registration of propidium iodide (PI)-stained Thy1-YFP-H mouse brain images

From: descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

 

Nuclear staining channel

Target channel

ANTs

SyN registration

Apply transform

Affine registration

Apply affine transform

SyN registration

Apply transform

Channel

Nuclear

staining

Nuclear

staining

Nuclear

staining

Target

Nuclear

staining

Target

Resolution

Downsize (50%)

Full size

Downsize (50%)

Downsize (50%)

Downsize (50%)

Full size

FOV1

5 h 53 m 10 s

11 m 39 s

7 m 53 s

56 s

5 h 46 m 30 s

10 m 19 s

FOV2

5 h 53 m 50 s

12 m 43 s

7 m 01 s

1 m 02 s

5 h 52 m 30 s

11 m 22 s

FOV3

5 h 53 m 30 s

14 m 05 s

9 m 43 s

1 m 12 s

5 h 46 m 30 s

12 m 26 s

FOV4

5 h 53 m 40 s

12 m 11 s

12 m 52 s

1 m 02 s

5 h 46 m 50 s

11 m 10 s