Fig. 5: Structure of the human mitochondrial CCA-adding enzyme maturing tRNAIle.
From: Structural basis for human mitochondrial tRNA maturation
a Cryo-EM density map and cartoon representation of the structure of TRNT1 poised to add the first cytosine to tRNAIle, with the color code of Fig. 1a, b. b Vacuum electrostatics surface representation of TRNT1 in the TRMT10C(SAH)/SDR5C1(NADH)/tRNAIle/TRNT1 structure. Positive charges are in blue, whereas negative ones are in red with the maximum color saturation corresponding to −5 kT/e (red) and +5 kT/e (blue). tRNAIle is represented as red ribbon and sticks. The different domains of TRNT1 are indicated. c Cryo-EM density map showing the stacking of A73 of tRNAIle with the CDP in the TRNT1 active site and base-specific interactions between TRNT1 and CDP. Potential hydrogen bonds are shown as dashed black lines. The residues of the catalytic site are represented as beige sticks. d, Interaction between TRNT1 (in beige), the T-loop of tRNAIle (in red) and TRMT10C (in green). e Interactions between the body and tail domains of TRNT1 (in beige) and tRNAIle (in red).
