Fig. 5: Loss of MIG-14/Wntless, EGL-20/Wnt and CAM-1/Ror disrupts asymmetric localization of TWK-28. | Nature Communications

Fig. 5: Loss of MIG-14/Wntless, EGL-20/Wnt and CAM-1/Ror disrupts asymmetric localization of TWK-28.

From: Wnt-Ror-Dvl signalling and the dystrophin complex organize planar-polarized membrane compartments in C. elegans muscles

Fig. 5

A Schematic protein structure of DSH-1a including DIX, PDZ, and DEP domains. B Muscle-specific expression of full-length or DIX domain-truncated DSH-1a proteins rescues TWK-28 localization in dsh-1(ok1445). PDZ and DEP domains are necessary for DSH-1 function in muscle polarity. C Loss of MIG-14/Wntless, EGL-20/Wnt, and CAM-1/Ror disrupts polarized localization of TWK-28. Posterior mid-body muscle cells are affected in all mutant genotypes. In cam-1 and egl-20 mutants, polarity is not affected in muscle cells situated anteriorly to the vulva (“Anterior midbody”). D Schematic protein structure of CAM-1/Ror receptor, with corresponding molecular lesions in point mutants (gm122 and cw82), and deletion alleles (ak37 and ks52). E cam-1(ak37), cam-1(ks52), and cam-1(cw82) disrupt TWK-28 polarity in posterior mid-body muscles. Left- or right-pointing yellow arrowheads indicate anterior or posterior extremity of muscle cells, respectively. Non-specific intestinal lysosome-related autofluorescence is visible in some panels and labelled with a white asterisk. Scale bars, 20 μm.

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