Fig. 6: DSH-1/Dishevelled is required to establish and maintain muscle polarity. | Nature Communications

Fig. 6: DSH-1/Dishevelled is required to establish and maintain muscle polarity.

From: Wnt-Ror-Dvl signalling and the dystrophin complex organize planar-polarized membrane compartments in C. elegans muscles

Fig. 6

A Representative micrograph of an mNeonGreen translational knock-in line of DSH-1. DSH-1 is enriched in the posterior portion of body wall muscle cells. DSH-1-mNeonGreen is visible in ventral nerve cord motoneuron cell bodies and neurites. Ventral view. B DSH-1-mNeonGreen distribution in a single muscle cell. Muscle membrane labelled by TWK-12-wrmScarlet (in magenta). Average position of DSH-1 domain boundary, 72 % of muscle cell length, n = 29. Each data point represents one muscle cell. N = 3 independent experiments. Ant, anterior; Pos, posterior. Grey bar indicates the region containing DSH-1. C DSH-1 and TWK-28 are found at opposite ends of muscle cells. Dashed lines indicate the outline of muscle cell. D Asymmetric localization of TWK-28 in L1, L2, and L3 larval stages. Non-polarized muscle cells are found in wild type at the L1 stage. Loss of dsh-1 disrupts muscle polarity at all stages. Muscle cells are labelled with cytoplasmic mCherry in the L1 stage and outlined with white dotted lines. E DSH-1 is required to maintain muscle polarity during post-embryonic development. F Muscle polarity is restored by re-expression of DSH-1 during post-embryonic development. Left- or right-pointing yellow arrowheads indicate anterior or posterior extremity of muscle cells, respectively. Scale bars, 20 μm. Raw data are presented in the Source Data file.

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