Fig. 4: Discovery and characterization of the sis BGC family. | Nature Communications

Fig. 4: Discovery and characterization of the sis BGC family.

From: Rule-based omics mining reveals antimicrobial macrocyclic peptides against drug-resistant clinical isolates

Fig. 4: Discovery and characterization of the sis BGC family.

a The sis BGC, structure of sistertide A1 and suggested structure of sistertide A2, and antibacterial potential of S. albus/pCZSIS crude extract. The AviMeCys motif is shown in pink. D-amino acids are shown in red. Other noncanonical amino acid are shown in blue. b The keb BGC. c UPLC-HRMS analysis of Streptomyces kebangsaanensis DSM42048 wild-type strain (i), S. albus/pCZKEB (ii, heterologous expression of keb BGC) and S. albus/pJTU2554 (iii, empty vector). d Suggested structures of kebanetides. e Bioactivity of kebanetides against M. luteus. f Chimeric precursor with the maturase from keb BGC. g UPLC-HRMS analysis of new-to-nature analog from construct keb-syn2. Source data are provided in MassIVE (https://doi.org/10.25345/C5PC2TM3Q).

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