Fig. 5: Immunization of mice with ΔmenT4ΔT3 results in increased antigen-specific T_H1-response.

a–f C57BL/6 mice immunized with saline or BCG or ΔmenT4ΔT3 were sacrificed at 6 weeks post-immunization, and intracellular cytokines were measured in PPD-stimulated splenocytes. a Representative FACS plots depicting percentage frequency of CD4⁺ IFN-γ⁺ and CD4⁺ T-bet⁺ T cells in spleens of naive or BCG or ΔmenT4ΔT3 immunized mice. These panels show mean ± SD of percentage frequency CD4⁺ IFN-γ⁺ (b) and CD4⁺ T-bet⁺ (c) in spleens of naive or BCG or ΔmenT4ΔT3 immunized mice. d Representative FACS plots depicting percentage frequency of CD8⁺ IFN-γ⁺ and CD8⁺ T-bet⁺ T cells in spleens of naive or BCG or ΔmenT4ΔT3 immunized mice. These panels show the percentage frequency of CD8⁺ IFN-γ⁺ (e) and CD8⁺ T-bet⁺ (f) T cells in the spleens of naive or BCG or ΔmenT4ΔT3 immunized mice. The data shown in (b, c, e, f) are mean ± S.D. of the proportion of T-cells obtained from five animals from a single experiment. The levels of secreted IFN-γ (g), TNF-α (h), IL-2 (i), IL-17A (j) were measured in the culture supernatants from PPD-stimulated splenocytes by ELISA. The data shown are mean ± SD of cytokine levels in PPD-stimulated splenocytes obtained from five animals from a single experiment (except in (h), BCG n = 4). p values depicted on the graphs were assessed using one-way ANOVA. Source Data are provided as a Source Data file.