Fig. 5: Multimodal control of bacterial gene expression by red and blue light. | Nature Communications

Fig. 5: Multimodal control of bacterial gene expression by red and blue light.

From: Leveraging the histidine kinase-phosphatase duality to sculpt two-component signaling

Fig. 5

a Schematics of the red-light-responsive DmDERusk circuit, developed at present, and of the earlier pAurora system that reacts to blue light45. b, c Bacteria harboring both pAurora-DsRed and DmDERusk-YPet were incubated under different red and blue light intensities. The YPet (b, green) and DsRed (c, pink) fluorescence readings were normalized to the optical density of the bacterial cultures at 600 nm (OD600) and corrected for background fluorescence. Data represent the mean of n = 3 biologically independent replicates. Light intensities are averaged over the duty cycle as marked by angled brackets. Source data are provided as a Source Data file.

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