Fig. 5: Shed cell walls and red bodies can be collected from the media.
From: Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan
A 100 mL of culture was concentrated into 1 mL by centrifugation, showing the red sediment on top of the cell pellet (cyan arrowhead). B, C This sediment imaged by fluorescence microscopy shows elongated, pointed particles (presumably shed cell walls) and smaller, autofluorescent spherical particles (presumably shed red bodies). Differential interference contrast (DIC) transmitted light and green autofluorescence (GAF) acquired as described previously. D, E, F Whole mount, negative stained, transmission electron microscopy of the red sediment, showing the spherical shed red bodies wrapped in the shed cell wall (D, E) or alone (F). G, H, I Resin-embedded, thin section samples of concentrated culture showing an elongate particle resembling the outer algaenan layer of a nearby intact cell (G), an apparent cross-section of a shed wall and putative red body (H), and this same particle imaged at higher magnification showing a relatively homogenous interior of moderate electron density (I).
