Fig. 4: The deficiency of PR-SET7 led to reduced number and derailed differentiation of inner stromal cells. | Nature Communications

Fig. 4: The deficiency of PR-SET7 led to reduced number and derailed differentiation of inner stromal cells.

From: PR-SET7 epigenetically restrains uterine interferon response and cell death governing proper postnatal stromal development

Fig. 4

a Diagram illustrating uterine heterogeneity in Pr-set7f/f and Pr-set7d/d mice on PND15. b UMAP plot showing cell clusters in Pr-set7f/f and Pr-set7d/d uteri on PND15. c The percentage of stromal subsets in Pr-set7f/f and Pr-set7d/d uteri on PND15. d SCRINSHOT analysis of Wnt16 and Htra3 in the Pr-set7f/f and Pr-set7d/d uteri on PND15. Dash lines represent the boundary between the epithelium and the stroma, as well as the stroma and the myometrium. Scale bar: 100 μm. e IF analysis of PR in Pr-set7f/f and Pr-set7d/d uteri on PND15. Dash lines represent the boundary between the epithelium and the stroma, as well as the stroma and the myometrium. Scale bar: 100 μm. f IHC staining of PR in 4-week-old Pr-set7f/f and Pr-set7d/d uteri. Dash lines represent the boundary between the stroma and the myometrium. Scale bar: 100 μm. g Scatter plot showing the differentially expressed genes in the Pr-set7f/f and Pr-set7d/d inner and outer stroma. h GO functional analysis of the upregulated and downregulated genes in the inner and outer stromal cells due to PR-SET7 loss. Significance is based on over-representation analysis with clusterProfiler. i Violin plot indicating the expressions of Prdm1 and Gli1 in the Pr-set7f/f and Pr-set7d/d inner stroma. j UMAP visualization indicating the expression of Ihh in Pr-set7f/f and Pr-set7d/d uteri. k CellChat analysis showing the ncWNT signaling pathway among different cell clusters in Pr-set7f/f and Pr-set7d/d uteri.

Back to article page