Fig. 6: Comparison of different gTBEs. | Nature Communications

Fig. 6: Comparison of different gTBEs.

From: Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase

Fig. 6

a The strategies for protein engineering and screening used in three studies. b Schematic of the basic architectures for various base editors. The bipartite nuclear localization signal (bpNLS) is shown in dark gray, linker in light gray, and nCas9 in teal green. UNG2* (in light green), UNG2 variant from the corresponding base editor. ΔNTD, deletion of the N-terminal domain. c The frequencies of T conversions at 17 endogenous loci. The thymines with editing frequencies > 25% for any base editors were showed. The highest frequencies at corresponding positions were highlighted as Heat map (n = 3 independent biological replicates per site. Note n = 2 for site 44 targeted by gTBEv4.). d Frequencies of T conversions by various base editors across the protospacer positions 1–20 (where PAM is at positions 21–23) from the edited sites in (c). Single dot represents individual replicate, and boxes span the interquartile range (25th to 75th percentile); horizontal lines within the boxes indicate the median (50%); and whiskers extend to the minimal and maximal values. Source data are provided as a Source Data file.

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