Fig. 4: Fumarate mapping in tissue section.
From: Raman micro-spectroscopy reveals the spatial distribution of fumarate in cells and tissues
A Top: Raman intensity maps for the 1130 cm−1 band, used to show the outline of the mouse kidney tissue section. Bottom: Fumarate concentration maps obtained by fitting the 1401 cm−1 band. The white outline traced from the Raman intensity maps serves as a guide to the eye. B Relative occurrence of fumarate concentrations in each area scan, normalised by scan size. Measured concentrations likely vary from true concentrations due to variations in focussing over the specimen, however, Fh1fl/fl tissues can be distinguished from induced tissues Fh1−/− by whether the majority of calculated concentrations are above the LOD. Source data are provided as a Source Data file.
