Fig. 5: Single-cell water spectroscopy.
From: Probing the structure of water in individual living cells
a Distributions of total protein concentration, amount of biointerfacial water (molar), weight of biointerfacial water (%, as compared to intracellular water) in different cell lines. b Heterogeneity of three parameters presented in a. Coefficient of variation (CV) values for each parameter were calculated as the ratio of the standard deviation to the mean for all cells including HeLa (57 cells), Macrophage RAW 264.7 (18 cells) and HT22 (28 cells). Total sample size n = 103. Error bars represent the minimum and maximum of values. c Plot of tetrahedral order parameter as a function of biointerfacial water concentrations, showing the invariance of water tetrahedrality (mean ± s.d. is 0.56 ± 0.01). d Positive correlation between the intensities of the two main peaks in biointerfacial water spectra. e, f Calculated thickness of biointerfacial water in different cell lines based on the whole-cell model. HeLa: D = 2.6 ± 0.6 (Å). RAW 264.7: D = 2.7 ± 0.7 (Å). HT22: D = 2.7 ± 0.5 (Å).
