Fig. 3: Positions of DSB.
From: Uncovering the dynamics of precise repair at CRISPR/Cas9-induced double-strand breaks
A–C Proportion of total DSBs (normalized to total molecules obtained in the in vivo time course) plotted by position along the target sequence with the expected cut site between -3 and −4 bp upstream of the PAM site, (A) Psy1, (B) CRTISO and (C) PhyB2. Bars indicating the proportion of DSBs captured at different positions over all time points of the time courses (n = 14) are shown with different colours based on the position, as shown in the legend. The positions of DSB for individual time points of the time courses are shown in Fig.S3. D-E Proportion of DSBs induced in vitro (normalized to total DSBs) plotted by the position of the captured end at (D) Psy1, (E) CRTISO, and (F) PhyB2; (n = 4).
