Fig. 4: A 3-state model of DSB induction and repair.
From: Uncovering the dynamics of precise repair at CRISPR/Cas9-induced double-strand breaks
A schematic and equations for a 3-state model of DSB induction Kcut in red, precise repair, Prepair in green and Error-prone repair Erepair, in blue. B–D Predicted fit of the model (lines) at Psy1 (B), CRTISO (C), and PhyB2 (D) for Intact molecules (green), DSB (red), and indels (blue). Confidence intervals are shown as shadings and calculated from 100 bootstraps of the data. Observed data are represented as dots. E–G Rate constants estimated at Psy1 (E), CRTISO (F), and PhyB2 (G) in terms of number of events per hour per molecule. The smoothed distribution of the estimates obtained through the bootstrap procedure is shown as a violin plot. Mean estimate is shown as a black point. Grey points represent 100 instances of stratified bootstrap. (see related Table 1, Fig. S2, Table S1).
