Fig. 5: Characterizing putative intermediates of DSB repair.
From: Uncovering the dynamics of precise repair at CRISPR/Cas9-induced double-strand breaks
A Schematic of categories of DSB types: Direct DSB (−3 bp from the PAM site), Processed DSBs (putative repair intermediates) classified as Guide, PAM, and Extended DSBs. B–D Percent of DSB types along the time-course of repair, for Psy1 (B), CRTISO (with perfect DSBs including both -3 and −4 bp) (C) and PhyB2 (D). Direct DSBs are shown in blue, guide-side DSBs in yellow, PAM-side DSBs in green and extended DSBs with extensions that do not match the target sequence in red.
