Table 2 Rates and flow estimates for the 3-state model of DSB Repair for the 24 h high-resolution time course
From: Uncovering the dynamics of precise repair at CRISPR/Cas9-induced double-strand breaks
Target | Process | Rate (proportion of total event/hour) | CI [1–99%] | Flow after 24 h (proportion of total molecules) | CI [1-99%] | P-value |
|---|---|---|---|---|---|---|
Psy1 | Cutting | 0.0164 | 0.0139-0.0217 | 0.3439 | 0.2828-0.4287 | <0.01 |
Repair-error | 0.0777 | 0.0666-0.0929 | 0.1348 | 0.1193-0.1555 | <0.01 | |
Precise Repair | 0.0691 | 0.028-0.131 | 0.1198 | 0.0491-0.2207 | <0.01 | |
e (processed DSB) | 0.1228 | 0.1012-0.1421 | / | / | <0.01 | |
U (uncut fraction) | 0 | 0–0.0031 | / | / | 0.97 | |
r (induction speed) | 12336.24 | 116.261-22425.4293 | / | / | 0.01 | |
d (induction decay) | 0 | 0–0 | / | / | <0.01 | |
Repair accuracy | / | / | 0.4706 | 0.2336-0.6193 | <0.01 | |
CRTISO | Cutting | 0.0251 | 0.021-0.0299 | 0.4339 | 0.3808-0.4795 | <0.01 |
Repair-error | 0.0538 | 0.0445-0.082 | 0.197 | 0.1767-0.2457 | <0.01 | |
Precise Repair | 0 | 0–0.0187 | 0 | 0–0.0598 | 0.93 | |
e (processed DSB) | 0.1016 | 0.0779-0.1133 | / | / | <0.01 | |
U (uncut fraction) | 0 | 0–0.0043 | / | / | 0.97 | |
r (induction speed) | 10.1122 | 8.1819-11.9085 | / | / | <0.01 | |
d (induction decay) | 0 | 0–0.0176 | / | / | <0.01 | |
Repair accuracy | / | / | 0 | 0–0.2371 | 0.93 | |
PhyB2 | Cutting | 0.0624 | 0.0537-10 | 0.41 | 0.1718-194.3107 | <0.01 |
Repair-error | 0.0274 | 0.0191-0.0334 | 0.1195 | 0.0568-0.1541 | <0.01 | |
Precise Repair | 0.0372 | 6e-04-55.8065 | 0.162 | 0.0021-181.6375 | 0.01 | |
e (processed DSB) | 0.1455 | 0.1238-0.1645 | / | / | <0.01 | |
U (uncut fraction) | 0 | 0–0.3821 | / | / | 0.83 | |
r (induction speed) | 7838.409 | 0-33839.845 | / | / | 0.08 | |
d (induction decay) | 0.0922 | 0–1.375 | / | / | <0.01 | |
Repair accuracy | / | / | 0.5754 | 0.0272-0.9996 | 0.01 |
