Fig. 3: Electrical responses of fusion via early and late entry pathways recapitulated on host cell-derived SLB. | Nature Communications

Fig. 3: Electrical responses of fusion via early and late entry pathways recapitulated on host cell-derived SLB.

From: Recreating the biological steps of viral infection on a cell-free bioelectronic platform to profile viral variants of concern

Fig. 3

a The experimental group for early fusion pathway consisted of VPPSpike and an SLB containing ACE2 (green) and TMPRSS2 (yellow). EIS Signals are characteristic of fusion events showing the changes in SLB membrane resistance in the equivalent electrical circuit scenario; b the experimental group consisted of the VPPSpike and an SLB containing ACE2 (green) and CatL (navy), where signals are characteristic impedance data of fusion events (note: pink color = acidic environment); c distribution of membrane resistance changes at all events of all systems (3 biological replicates for all systems, n = 9 for reversed early fusion and n = 8 for all other systems) using Spike protein from SARS-CoV-2 Wuhan-Hu-1. ΔR data are mean ± SD; statistical analysis was performed using one-sided one-way analysis of variance (ANOVA) with Šidák’s multiple comparisons test.

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