Fig. 1: UBE2D3 is a regulator of telomere-driven genomic instability.
From: UBE2D3 facilitates NHEJ by orchestrating ATM signalling through multi-level control of RNF168
a Outline of the screen performed to identify factors controlling telomere-driven genomic instability. b Survival assays after 2 weeks of telomere uncapping for 2 independent Ube2d3 shRNAs and 1 Nbs1 shRNA. Representative plates from 8 independent experiments for control and Ube2d3 sh1 and 2 independent experiments for Ube2d3 sh2 and Nbs1 sh are shown. Representative immunoblots for Ube2d3 depletion from 2 independent experiments are shown. c Left: Growth curves of control and Ube2d3 knockdown TRF2ts MEFs (clone C15) upon induction of telomere uncapping (n = 3 independent experiments; mean ± SEM; two-tailed Student’s t-test). Immunoblot for UBE2D3 in cells used for growth assays are shown below (R1, R2, R3 = replicate 1, 2, 3). Right: Representative photographs of arrested and dying control TRF2ts MEFs and proliferating Ube2d3 knockdown TRF2ts MEFs after 12 days of telomere uncapping (TRF2ts clone B17). Scale bar represents 10 μm. Growth curves from these cells can be found in Supplementary Fig. 1c. d Survival assays upon Ube2d1, Ube2d2 and Ube2d3 depletion, after 12 days of telomere uncapping and at 32 °C. Representative plates from 2 independent experiments are shown. Source data are provided as a Source Data file.
