Fig. 3: The exacerbation of ILC2-mediated airway inflammation due to SFR-deficiency is dependent on TH2 response.

Detection of CD4⁺ T cells (a) and eosinophils (b) in DMSO (control) or FTY720-treated mice at day 9 following papain treatment (i.n., day 0 to 2). n = 4, 3, 4, 4 mice (left to right groups). c The number of eosinophils in mice at day 9 following papain treatment (i.n., day 0 to 2). n = 3, 3, 6, 5 mice (left to right groups). d The number of eosinophils on day 9 in the papain-induced (i.n., day 0 to 2) mice depleted of CD4⁺ T cells. α-CD4, anti-CD4 antibody; control, IgG1. n = 5 (WT) and 3 (SFR^−/−) mice per group. e The numbers of TH2 cells (CD3⁺CD4⁺GATA-3⁺) in the papain-induced (i.n., day 0 to 2) mice on day 9. n = 4 mice per group. f The correlation analysis between the numbers of TH2 cells and eosinophils in the lungs from WT and SFR^−/− mice at day 9 following papain treatment (i.n., day 0 to 2). n = 20. g The number of CD69⁺CD4⁺ T cells in the papain-induced (i.n., day 0 to 2) mice on day 9. n = 3 mice per group. The proportion and number of tetramer-traceable CD4⁺ T cells on day 9 after papain plus peptide 2W1S (h) or peptide OVA323 (i) treatment (i.n., day 0 to 2). n(WT) = 5 (h) or 3 (i) mice; n(SFR^−/−) = 3 (h) or 4 (i) mice. j Detection of indicated cells in mice at day 9 following peptide 2W1S plus papain treatment (i.n., 0 to 2). n = 5, 4, 8, 8, 3, 6, 4, 6, 5, 4, 8, 8 mice (left to right groups). k Detection of indicated cells in SR3335-treated mice at day 9 following peptide 2W1S plus papain treatment (i.n., day 0 to 2). n  = 4, 5, 5, 4 mice (left to right groups). The data represent at least three independent experiments with similar results. All data are represented as means ± SEM, and statistical analysis (±SEM) was conducted using one-way ANOVA (a–d, j, k), two-tailed Student’s t test (e, g–i), or linear regression (f). ns, not significant. Please refer to Supplementary Fig. 3 for further details.