Fig. 5: Foxf1 expression in pericytes is regulated by EBF1. | Nature Communications

Fig. 5: Foxf1 expression in pericytes is regulated by EBF1.

From: Identification of endothelial and mesenchymal FOXF1 enhancers involved in alveolar capillary dysplasia

Fig. 5

a The sequence alignment of the evolutionarily conserved FEL4 enhancers shows the EBF binding site. The EBF-binding motif is shown in the EBF-binding site located in the FEL4 enhancer. b EBF1 is the most highly expressed member of the Ebf family of transcription factors in E18.5 lung as shown by single cell RNA sequencing. c The dot plot shows a correlation analysis between Foxf1 and Ebf1 in pulmonary pericytes from E18.5 lungs. The percentage of Foxf1+Ebf1+ pericytes is 39.6%. d Disruption of the EBF1 binding motif significantly reduces the activity of the FEL4 enhancer after co-transfection with CMV-Ebf1 plasmid, in the dual luciferase assay, and n = 4 biological replicates. Data are presented as mean ± SD. p < 0.01 is **p < 0.05 is *, ns is not significant. Two-sided Student’s t test is used for statistical analysis, and p-value is 0.01096. Source data are provided as a Source Data file.

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