Fig. 3: Molecular basis of RAI2-induced CtBP polymerization. | Nature Communications

Fig. 3: Molecular basis of RAI2-induced CtBP polymerization.

From: Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2

Fig. 3

a Cryo-EM density (left), schematic (middle), and cartoon representation of the CtBP1(WT, FL)/RAI2(Δ11, 303–362) filament confined to six CtBP1 tetramer layers (right) that are colored in cyan, violet, and light blue. Adjacent CtBP1 layers are rotated around a vertical 3-fold filament axis and are connected by a pair of RAI2 peptides (yellow) with an ALDLS tandem motif (orange) in opposite positions to the central filament axis. Book-like opening of a CtBP1(n)/CtBP1(n + 1) interface (boxed) without bound RAI2 is indicated schematically. b Top view of the RAI2-mediated CtBP1(n)/CtBP1(n + 1) interface in cyan and pale cyan (boxed in a, left). Areas interacting with the next tetrameric CtBP layer are in blue and dark blue. The four CtBP subunits are numbered as in Fig. 2e. Residues involved in specific CtBP1/CtBP1 interface interactions are indicated (cf. c). RAI2-interacting surface segments are in orange (ALDLS motif) and yellow (other residue segments). c Side view of the CtBP1(n)/CtBP1(n + 1) interface (boxed in a, left), highlighting secondary structural elements and residues that contribute to specific interface interactions (cf. Supplementary Fig. 13). d Side view of the visible RAI2 segment interacting with two adjacent CtBP layers (boxed in a, right). The connecting RAI2 tandem ALDLS linker (yellow) is in close contact with a third CtBP subunit (violet). See also Supplementary Figs. 10–14 and Supplementary Tables 3 and 4.

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