Fig. 1: M-833 resistant parasites contain mutations in the P. falciparum START1 protein. | Nature Communications

Fig. 1: M-833 resistant parasites contain mutations in the P. falciparum START1 protein.

From: Aryl amino acetamides prevent Plasmodium falciparum ring development via targeting the lipid-transfer protein PfSTART1

Fig. 1

A Structure of MMV006833 (M-833). B Parasites populations (Pop) resistant to M-833 were generated by exposing 3D7 P. falciparum parasites to 10 x EC50 of M-833 three times. These parasites were cloned, and growth inhibition assays performed in the presence of 10 µM to 0.04 µM M-833. Growth inhibition was normalised to DMSO controls. Average of EC50 values and 95% confidence intervals are indicated; n = 3 biological replicates. Source data are provided as a Source Data file. C Key domains and cartoon representation of the AlphaFold predicted structure of the StAR-related lipid transfer protein, PfSTART1 (PF3D7_0104200)70,71. Mutations that had been identified in the M-833 resistant parasite populations are shown as sticks (yellow = N309K, N330K; green = I224).

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