Fig. 3: Spontaneously formed ALK fusions drive tumor formation in vivo.
From: Mechanistic patterns and clinical implications of oncogenic tyrosine kinase fusions in human cancers
a Experimental timeline of the in vivo experiment in NSG mice grafted with BEAS-2B cells. b Tumor growth in NSG mice grafted with BEAS-2B cells. DNA DSBs in intron 6 of EML4 and intron 19 of ALK (red, n = 4), in intron 13 of EML4 and intron 19 of ALK (blue, n = 4), or in intron 19 of ALK (black, n = 40). c Percentage of ALK-fusion positivity in tumors grown in NSG mice grafted with BEAS-2B cells. d Validation of the indicated ALK fusion transcripts from tumors developed in NSG mice. Similar results were obtained from n = 2 independent experiments. e, f Representative western blots (e) and immunohistochemistry (f) from tumors grown in mice grafted with BEAS-2B cells introduced DNA DSBs only in intron 19 of ALK. Scale bar = 50 μm. Similar results were obtained from n = 2 independent experiments. Source data are provided as a Source Data file.
