Fig. 4: Genetically encoding eFSY into proteins in mammalian cells. | Nature Communications

Fig. 4: Genetically encoding eFSY into proteins in mammalian cells.

From: Characterize direct protein interactions with enrichable, cleavable and latent bioreactive unnatural amino acids

Fig. 4

a Fluorescence images of cells expressing EGFP(Y151eFSY), showing successful incorporation of eFSY into EGFP in HEK293T cells. b Intact protein mass analysis of EGFP(D190eFSY) expressed in HEK293T cells. c Tandem mass spectrum of eFSY incorporated peptide derived from EGFP(D190eFSY). d Western blot analysis of HEK293T cell lysates expressing GST(T103U), showing cross-linking of GST dimer in live cells. Source data are provided as a Source Data file. e Identified inter-chain cross-links mapped onto the protein structure of GST (PDB ID 1A0F). The eFSY incorporation site was shown in magenta sticks. The cross-linking sites were shown in orange sticks. f–h Tandem mass spectra of inter-chain cross-linked peptides derived from GST.

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