Fig. 7: Efficient yeast cell factory for biosynthesis of halogenated BIA derivatives.

a LC–QQQ traces displaying peaks of halogenated BIA derivatives after feeding 0.5 mM halogenated tyrosine. Specific MS² spectra detected through production mode and potential characteristic fragments are detailed in Supplementary Fig. 27-31. High-resolution MS² spectra captured by LC-MS-TOF are presented in Supplementary Fig. 32-41. b Comparison of LC–QQQ peaks and MS² spectra between protopine standard and F-protopine. c Comparison of LC–QQQ peaks and MS² spectra between sanguinarine standard and F-sanguinarine. d High-resolution MS spectra of F-protopine detected by LC-MS-TOF, with possible fragment formulas detailed in Supplementary Fig. 40. e High-resolution MS² spectra of F-sanguinarine by LC-MS-TOF, with possible fragment formulas detailed in Supplementary Fig. 41. Source data are provided as a Source Data file.