Fig. 6: Rescue of respiration deficient phenotype by YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3 -SynYLG6 chimeras expressing a glf and invA protein.
From: Introducing carbon assimilation in yeasts using photosynthetic directed endosymbiosis
A Low levels of growth for YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3 under glycerol as the carbon source. Nfu1: Protein involved in Fe-S cluster transfer to the mitochondrial proteins; Isu1: performs a scaffolding function during the assembly of iron-sulfur clusters; Mis1: Mitochondrial C1-tetrahydrofolate synthase. The figure was prepared using BioRender. B Growth of S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3-SynYLG6-chimeras on selection medium. The experiment was repeated three times independently with similar results and as listed the respective P-values are <0.0001, <0.0001 and 0.002. C Growth trends of S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3 (yeast only strain), S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3-SynJEC3, S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3-SynYLG6 chimeras. Cells (3.00 × 103) from each round were spotted on subsequent selection medium as described in the methods section and the final number of cells/spot on plate were determined after 48 h of growth (n = 5 technical replicates; data are presented as mean values + /− SEM). D Total DNA of the yeast-SynJEC3/SynYLG6 chimeras contains yeast MATα and SynJEC3/SynYLG6 CAT genes. The experiment was repeated three times independently with similar results. E Cell count analysis to demonstrate the effect of (3-(3,4-dichlorophenyl) −1,1-dimethylurea), DCMU, on S. cerevisiae YPH500 nfuΔ::LEU2 isu1Δ::URA3 mis1Δ::HIS3-SynYLG6 chimeras (n = 5 technical replicates; data are presented as mean values + /− SEM). P-values were calculated by a two-tailed t-test comparing the two means. As listed the P-values for No DCMU and 20 µM DCMU treatment was <0.0001, for No DCMU and 40 µM DCMU was <0.0001 and 20 µM DCMU and 40 µM DCMU was 0.0004. F pTIRF microscopic images of the chimeric cells that were grown under selection conditions (Ex. = 561 nm: Em. = 653/95 nm). Panels are merged images of pTIRF (green) and brightfield microscopy (gray). The experiment was repeated twice independently (n = 2) with similar results. G Fluorescence confocal microscopy images of the chimeric cells, which were grown under selection conditions. The experiment was repeated twice independently (n = 2) with similar results. Source data are provided as a Source Data file.
