Fig. 2: Sex-specific effects of PR on aspects of energy balance in 3xTg mice.

Metabolic chambers were used to determine fuel source utilization, energy expenditure, and spontaneous activity over 24 h in female (A–C) and male (D–F) 6-month-old 3xTg and NTg mice fed Control or PR diets for 3 months. Respiratory exchange ratio (RER) in females (A) and males (D). Energy expenditure normalized to body weight in females (B) and males (E). Spontaneous activity of females (C) and males (F). A–C n = 5 biologically independent mice per group, data from female Control and PR NTg mice are plotted with gray and yellow bars respectively and data from Control and PR 3xTg mice are plotted with blue and pink bars. D–F n = 5 Control-fed NTg, 5 PR-fed NTg, 4 Control-fed 3xTg, and 4 PR-fed biologically independent mice per group. Data from male NTg Control and PR mice are plotted with blue and purple bars respectively and data from 3xTg Control and PR mice are plotted with fuchsia pink and coral pink bars. A–F Statistics for the overall effects of genotype (GT), diet, and the interaction represent the p value from a 2-way ANOVA conducted separately for the light and dark cycles, *p < 0.05, from a Sidak’s post-test examining the effect of parameters identified as significant in the 2-way ANOVA. Data represented as mean ± SEM. Source data is provided as a Source Data file.